|
KMID : 0379520010170030181
|
|
한국독성학회지
2001 Volume.17 No. 3 p.181 ~ p.186
|
|
|
Vitellogenin mRNA Induction in Male Carp Treated with Bisphenol A
|
|
Park Eung-Roh
Choi Pil-Son
Lee Chul-Woo
Ryu Ji-Sung
Nam Seung-Sook
Rhu Hong-Il
Jeon Seong-Hwan
Na Jin-Gyun
Park Kwang-Sik
|
|
|
Abstract
|
|
|
|
The estrogenic potency of bisphenol A using reverse trancriptase-PCR response of liver vitellogenin mRNA in male carp was studied. For this, six combination of primers which were synthesized on the basis of cDNA consensus region of various species, were evaluated and one pair of primers was selected as the best to show 286 bp size-transcript. By using the selected primers, vitellogenin mRNA induction in carp treated with bisphenol A was measured and the chemical showed dose-and time-dependent Induction response. From this result, it was concluded that RT-PCR technique wing the selected primers in this study can be wed to monitor the estrogenic effects exerted In carp living in Korean freshwater.
|
|
|
KEYWORD
|
|
|
Carp, Vitellogenin, RT-PCR, Bisphenol A
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|